Our acquity uplcms sample organizer, sample manager, bsm, pda and sqd is running on windows 7 with masslynx 805. Overview of waters acquity sample organizer video labtube. Acquity uplc beh columns can tolerate pressures of up to 15,000 psi 1034 bar or 103 mpa. The acquity uplc system is to be used for in vitro diagnostic use only by trained, qualified laboratory personnel. Plumbing the binary solvent manager and sample manager.
The waters acquity uplc mclass system is a nano to microscale ultraperformance lc r uplcr system rated for 15,000 psi operation that when coupled to waters mass spectrometers, delivers the. The sample organizer initializes and scans the shelves for their positions and also for which shelves contain plates. The uplcmsms system consisted of an acquity ultraperformance lc waters, milford, ma coupled to a 4000 qtrap lcmsms ab sciex, foster city, ca. The waters acquity uplc system is a robust uplc machine which we use regularly in our lab. December 20 720003294en lbpdf connections insight providesrealtime monitoring automaticnotification instrumentperformance diagnosticinformation allowing quickerproblem resolution local control acquity uplc local console controller lcc envi ronmental speci fi cati ons acoustic noise 65 dba, system operating temperature range. To reset the nonvolatile ram, enter service mode and then on the top line of the trouble shoot dropdown menu, select reset nonvolatile ram. Plates are transferred in a programmable order from the sample organizer to the acquity uplc system sample manager for processing.
We do not have ability to test the system but appear to be complete and operational. Plates and vials can be used in either the sample manager or sample organizer. Acquity, acquity hclass bio qsm, acquity hclass bio smftn, acquity iclass bsm, acquity uplc bsm, open architecture uplc, nanoacquity, nanoacquity uplc sample mgr. When operating the acquity uplc system, follow standard quality control procedures.
This morning i did an injection on vial a8 well plate 48 and i. The sample organizer will scan all the trays and sample plates. The acquity uplc sample organizer increases the level of automation and sample processing throughput of the acquity uplc system. L was chosen, because bigger volumes imply wider peaks without much enhancement of the signalto. Waters acquity uplc hclass system guide attachments waters acquity uplc hclass system guide 2 mb. Waters designed the acquity uplc system to isolate, concentrate. The acquity uplc hclass system is the only quaternary lc system in the market with a wide range of detectors that have been optimized for true uplc performance. This is a good compromise between analyte solubility and peak shape. Mve cryosystem 2000 sample storage with roller base and new cryo gloves. Acquity uplc system operators guide waters corporation. Specify the project and a new window run sample appears.
Waters acquity sample manager uplc sample manager in. Acquity uplc binary solvent manager available part numbers. This uplc system features a wide range of flow rates, back pressures and linear velocities, with increased throughput with high efficiency so you get the most for your time and resources. The acquity uplc iclass system is available with a choice of column management option. Waters acquity sample manager uplc sample manager in watertown, ma, usa. Waters sample organizer uplc autosampler loader in watertown. The so supports a wide range of plates and vial holders, and depending on the types of plates. With unprecedented usability, the acquity uplc mclass system enables laboratories to succeed in analyzing compounds that are severely limited in amount or availability, even. Acquity uplc hclass system guide conquer scientific. Acquity uplc sample manager problem chromatography forum. When operating the acquity uplc instruments, follow standard qualitycontrol qc procedures and the guidelines presented in this section. Significant advances in instrumentation and column technology were made to achieve dramatic increases in resolution, sensitivity and speed in liquid chromatography. Waters introduces uplc system for nano to microscale. The waters sample manager fixedloop smfl offers lowest dispersion performance and is suitable for running 1.
Applicable symbols audience and purpose this guide is intended for personnel who install, operate, and maintain acquity uplc instruments. See acquity uplc i class systems specification guide for conditions sample organizer sample plate capacity. Columns and consumables acquity uplc columns by waters. Applicable symbols audience and purpose this guide is intended for personnel who install, operate, and maintain acquity uplc hclass instruments. Choose the serial number of the sample organizer and press ok. Page 12 care and use manual in the sample are acceptable. Acquity uplc mclass system from waters selectscience. The problem we have is that we take a new column and before we make the first injection we stabilize with 100% methanolthat is the mobile phase and the pressure starts at aproximately 2500 psi at 0,5 mlmin. Beh technology is a key enabler of the speed, sensitivity and resolution of uplc separations. For users of acquity uplc and uhplc instruments, systems and columns. Intended use of the acquity uplc system waters designed the acquity. Waters consumable part list official gimitec website. Does anyone have an electrical power backup module ups on standby for their lc system.
Acquity uplc system operators guide engineering umass. Waters acquity h series uplc system model upb pump model upa autosampler model upd diode array detector model upx column compartment still on the bench. When the optional solvent selection valve is installed, it is plumbed to solvent channel d, enabling solvent selections d1 through d6 in addition to a, b, and c a total of nine solvents to select from. It extends the capacity of the system by more than tenfold for samples in standard ansicompliant 96 or 384 well plates. Waters acquity uplc system waters acquity uplc system. If free amino acid analysis is required you will need to deproteinize the sample and also get rid of high mw polysaccharides, probably the best way to do this is pulp, dilute with water, filter and the ultra filter a small amount through an ultra filter device similar to the millipore ultra free 5,000 cutoff tubes. Development and validation of a uplc method for the. Get waters acquity uplc beh columns instruction manual. C onfigured based on the types and combinat ions of plates being used. In the sample organizer information window, click configure scan and store shelf layout. The acquity uplc mclass system delivers the most robust, reliable, and highest quality nano to microscale uplc separations to every laboratory performing lcms analyses. The analysis youve always desired for nano to microscale separations the acquity uplc mclass system delivers the most robust, reliable, and highest.
Open the empower pro and click on the run sample button. See how the acquity uplc hclass flowthroughneedle sample manager enables familiar hplc operation and uplc performance. Significant advances in instrumentation and column design enabl. Minimizing dispersion and optimizing the electronics and optical paths for the narrow uplc peaks yields the highest sensitivity and repeatability with the most accurate quantification. A tenyear anniversary video of the acquity ultraperformance lc uplc system, a technology that created a new industry standard for chromatographic performa. Waters uplc acquity instrument core system includes. Getting started with acquity uplc beh amide columns operating ranges 1.
Acquity uplc sample organizer operators overview and. C are recommended for operating acquity uplc beh columns in order to enhance selectivity, lower solvent viscosity and increase mass transfer rates. Qbd fashion barcelona feather sample book 49 pages 12 x 12 x 2 book. I do recommend getting a preventive maintenance plan with the. The pump can deliver gradients in eleven curve shapes linear, two step, four convex, and four concave. Ultraperformance liquid chromatography tandem mass. The waters acquity uplc system provides you with reproducible, dependable and data rich results for challenging separations. In the acquity uplc console, select sample organizer from the system tree. When throughput is what you need, the ac quity uplc sample organizer so increases the level of automation and sample processing throughput of the ac quity uplc hclass system. Yes, the acquity uplc sample organizer increases the level of automation and sample processing throughput of the. This contained tower uplc system holds a sample manager, column heater, binary.
Plates are transferred from the sample organizer to the acquity uplc sample manager in a programmable order, and any sample position can be accessed at any time. The acquity uplc system is cemarked according to the european union in vitro diagnostic device directive 9879ec. This option can be found in the sample manager under the control menu. Sample organizer maximum sample capacity total of up to 8,448 samples.
Waters, acquity hclass bio qsm hplc replacement parts. Gradient smartstart easily manage system volume when transferring methods between different lc. How to configure the sample organizer in an acquity uplc. The sample organizer goes up, and this saves bench space, but if the samples are in different placesholders then shuttling the plateshelders back and forth is a fact of life. The same issue arises when need to run stat samples. Acquity uplc bsm kits, pump acquity uplc column heatercooler pump acquity uplc column heater module pump acquity uplc column mgr pump acquity uplc high temperature column heater pump acquity uplc i2v bsm kits, pump acquity uplc pda lamps acquity uplc sample mgr autosampler, kits acquity uplc tuv lamps alliance accessories. Operating the acquity uplc hclass instruments when operating the acquity uplc hclass instruments, follow standard qualitycontrol qc procedures and the guidelines presented in this section. Applicable symbols audience and purpose this guide is intended for personnel who install, operate, and maintain acquity uplc hclass bio instruments.
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